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Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
Evaluation of the immunogenicity of Adenovirus-based SARS-S1-CoV-2 vaccines in BALB/c mice
تقييم رد الفعل المناعي للقاح مبتكر قائم على الادينو فايروس ومستهدف للجزء البروتيني السطحي لفايروس S1 سارس2
Subject
:
Faculty of Science
Document Language
:
Arabic
Abstract
:
The development of safe and effective vaccine against the severe acute respiratory syndrome-Coronavirus 2 (SARS-CoV-2) become a global priority due to the ongoing pandemic of Coronavirus disease 2019 (COVID-19). Additionally, the emergence of SARS-CoV-2 variants has generated a need for more alternative vaccines using different strategies and/or antigens. In this study, we developed and assessed the immunogenicity of a novel recombinant replication-incompetent SARS-CoV-2 vaccines based on adenovirus serotype 5. Our generated construct expresses the CD40-targeted subunit 1 (S1) of SARS-CoV-2 spike protein (rAd5-SARS2-S1/tlhCD40L), and as a control we generated a construct expressing S1 alone (rAd5- SARS2-S1-S1), and as other one expressing Green Fluorescent Protein (rAd5-GFP). The efficacy and immunogenicity of our vaccine candidates have been evaluated in BALB/c mice. Intramuscular immunization with two doses of rAd5-SARS2-S1/tlhCD40L and rAd5- SARS2-S1-S1 induced robust and sustained specific immunoglobulin G (IgG), neutralizing antibodies (nAbs), B-cell and T-cell immune responses compared to rAd5-GFP. Although, both vaccine candidates could elicit strong humoral and cellular immune responses, integration of the molecular adjuvant CD40L enhanced the SARS-CoV-2-specific levels of IgG, nAbs, and B-cell. Importantly, we demonstrated that rAd5-SARS2-S1/tlhCD40L and rAd5- SARS2-S1-S1 induced cross-reactive neutralizing antibodies against SARS-CoV-2 variants of concerns. These results show that a replication-deficient human adenovirus serotype 5-based vaccine that expresses the S1 subunit fused to human CD40 ligand (CD40L) (rAd5-SARS2-S1/tlhCD40L) could be an effective potential vaccine for COVID-19.
Supervisor
:
Dr. Wesam Hussain Abdulaal
Thesis Type
:
Doctorate Thesis
Publishing Year
:
1444 AH
2022 AD
Co-Supervisor
:
Prof. Anwar Mohammad Hashem
Added Date
:
Monday, February 27, 2023
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
نجوى ضيف الله الجهني
Aljehani, Najwa DifAllah
Researcher
Doctorate
Files
File Name
Type
Description
49055.pdf
pdf
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