Main Page
Deanship
The Dean
Dean's Word
Curriculum Vitae
Contact the Dean
Vision and Mission
Organizational Structure
Vice- Deanship
Vice- Dean
KAU Graduate Studies
Research Services & Courses
Research Services Unit
Important Research for Society
Deanship's Services
FAQs
Research
Staff Directory
Files
Favorite Websites
Deanship Access Map
Graduate Studies Awards
Deanship's Staff
Staff Directory
Files
Researches
Contact us
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Deanship of Graduate Studies
Document Details
Document Type
:
Thesis
Document Title
:
ISOLATION AND CHARACTERIZATION OF INDIGENOUS BACILLUS THURINGIENSIS STRAINS FOR BIOCONTROL OF AEDES AEGYPTI
عزل وتوصيف سلالات محلية من Bacillus thuringiensis للمكافحة الحيوية للبعوض المصري
Subject
:
faculty of science
Document Language
:
Arabic
Abstract
:
Bacillus thuringiensis (Bt) is a rod-shaped, Gram positive bacterium that produces insecticidal crystal proteins also known as δ-endotoxin during sporulation. Crystal proteins or Cry proteins are toxic to a wide range of insect pests such as lepidopteran insects, beetles and mosquitoes etc. Due to their high specificity, efficacy and safety, these Cry proteins have been successfully used as a biopesticide to control various insect pests for the last six decades. Significance of the Cry proteins has motivated the search for new strains of Bacillus thuringiensis from diverse habitats around the globe to identify new crystal proteins with unique target species. Our goal of the proposed project was to isolate and characterize the new endogenous Bt strains producing crystal protein with more potent mosquitocidal activity as compared to the currently available Bt strains, from different regions of Saudi Arabia. Soil samples were collected from various regions of Saudi Arabia and isolation of Bt strains was carried out using the selective sodium acetate medium and heat treatment method. Around 85 soil samples were processed and screened which resulted in the isolation of 3 Bt isolates. Biochemical tests and phylogenetic analysis based on 16S DNA gene sequencing revealed that the three bacterial isolates were Bacillus thuringiensis strains. A crystal-spore differential staining revealed the expression of crystal proteins in all the three new endogenous Bt isolates during the sporulation phase. Spore-crystal protein mixture of all the three Bt isolate was shown to possess insecticidal activity against the larvae of Aedes aegypti. Moreover, tests under light microscopy showed that crystal proteins are present in all three isolates and have a fatal effect on the Aedes aegypti mosquito larvae.
Supervisor
:
Dr. Mohammad H. Fakieh
Thesis Type
:
Master Thesis
Publishing Year
:
1441 AH
2019 AD
Co-Supervisor
:
Dr. Naser A. Alkenani
Added Date
:
Thursday, December 12, 2019
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
جهاد أحمد دين
Din, Jihad Ahmed
Researcher
Master
Files
File Name
Type
Description
45830.pdf
pdf
Back To Researches Page