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Document Details
Document Type
:
Article In Journal
Document Title
:
leishmania donouani is the only cause of visceral leishmaniasis in east africa previous descriptions of L.
الليشمانيا donouani هو السبب الوحيد لداء الليشمانيات الحشوي في شرق أفريقيا الأوصاف السابقة من L.
Subject
:
leishmania donouani is the only cause of visceral leishmaniasis in east africa previous descriptions of L.
Document Language
:
English
Abstract
:
Isoenzyme-based studies have identified 3 taxa/species/‘phylogenetic complexes’ as agents of visceral leishmaniasis in Sudan: L. donovani, L. infantum and ‘‘L. archibaldi’’. However, these observations remain controversial. A new chitinase gene phylogeny was constructed in which stocks of all 3 putative species isolated in Sudan formed a monophyletic clade. In order to construct a more robust classification of the L. donovani complex, a panel of 16 microsatellite markers was used to describe 39 stocks of these 3 species. All ‘‘L. donovani complex’’ stocks from Sudan were again found to form a single monophyletic clade. L. donovani ss stocks from India and Kenya were found to form 2 region-specific clades. The partial sequence of the glutamate oxaloacetate transaminase (GOT) gene of 17 L. donovani complex stocks was obtained. A single nucleotide polymorphism in the GOT gene appeared to underlie the isoenzyme classification. It was concluded that isoenzyme-based identification is unsafe for stocks isolated in L. donovani endemic areas and identified as L. infantum. It was also concluded that the name L. archibaldi is invalid and that only a single visceralizing species, Leishmania donovani, is found in East Africa.
ISSN
:
1432-1955
Journal Name
:
Parasitology
Volume
:
129
Issue Number
:
1
Publishing Year
:
1424 AH
2004 AD
Article Type
:
Article
Added Date
:
Tuesday, March 5, 2013
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
منال بكر جمجوم
Jamjoom, Manal Bakr
Researcher
Doctorate
mjamjoom@kau.edu.sa
Files
File Name
Type
Description
35109.pdf
pdf
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