Document Details

Document Type : Article In Journal 
Document Title :
Screening and production of antibacterial compound from Trichoderma spp. against human-pathogenic bacteria
Screening and production of antibacterial compound from Trichoderma spp. against human-pathogenic bacteria
 
Subject : Biological 
Document Language : English 
Abstract : This study focus on the production of antibacterial compound from Trichoderma spp. Screening of antibacterial activities in some Trichoderma spp. was investigated using CYS80 medium. Trichoderma reesei and Trichoderma viride were highly effective toward human-pathogenic bacteria tested. T. viride and T. reesei were separately applied on Sephacryl S-200 column. Column fractions No. 56 to 64 for T. viride and fractions No. 57 to 66 for T. reesei had inhibitory effect against the most pathogenic bacteria examined. T. reesei and T. viride Sephacryl S-200 fractions with antibacterial activity were analyzed by Gas chromatography-mass spectrometry (GC- MS). The product with highest peak (95%), using different libraries, was kojic acid. The yield of kojic acid crystals from T. reesei and T. viride Sephacryl S-200 fractions were 3 and 5 g/L, respectively. Physical analysis of kojic acid with respect to UV, IR, 1HNMR analysis and melting point was examined. The minimum inhibitory concentration (MIC) of kojic acid and augmentin, as control, against human-pathogenic bacteria were evaluated. Kojic acid and augmentin showed a similar time-killing kinetics with human-pathogenic bacteria. The level of kojic acid increased with decreased level of reducing sugar during the growth of T. reesei and T. viride suggesting that the enzyme system for the synthesis of kojic acid found in the cell of these fungi. 
ISSN : 1996-0808 
Journal Name : African Journal of Microbiology Research 
Volume : 5 
Issue Number : 13 
Publishing Year : 1432 AH
2011 AD 
Article Type : Article 
Added Date : Wednesday, September 28, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
رشاد محمد صالحSaleh, Rashad MohamedResearcherMaster 
صالح عبدالله كابليKabli, Saleh AbdullahResearcherDoctoratesakabli@yahoo.com
صالح محمد القرنيAl-Garni, Saleh MohamedResearcherDoctorateSaleh-895_4@hotmail.com
صالح أحمد محمدMohamed, Saleh AhmedResearcherDoctoratesaleh38@hotmail.com

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