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Document Details
Document Type
:
Article In Journal
Document Title
:
Purification and Characterization of Protopectinase Produced by Kluyveromyces marxianus
تقنية و توصيف انزيم البروتوبكثينيز المنتج بخميرة كليفيروميسي ماركسيانس
Subject
:
Purification and Characterization of Protopectinase Produced by Kluyveromyces marxianus
Document Language
:
English
Abstract
:
The crude protopectinase preparation obtained from Kluyveromyces marxianus cultures was partially purified by fractional precipitation with ammonium sulphate. At 65% ammonium sulphate, the most active fractionation was obtained. Further purification by gel filtration on CM-Sephadix C-50 followed by ion exchange chromatography on Sephadix G-75 yielded 4 peaks of protopectinase components. The second peak was the major one containing most of the recovered protein and all the protopectinase activity. The enzyme showed different hydrolytic activities on some protopectin sources. Characterization of the enzyme including, enzyme concentration, amount of substrate, pH and temperature of the reaction mixture was carried out. The enzyme was stable up to 50ºC and at pH range between 4 and 7. The enzyme activity was responded differently to the tested metal ions and some inhibitors. The amino acids composition of the enzyme showed a high proportion of glycine and moderate amounts of glutamic acid, alanine and leucine but poor contents of proline, cysteine and tyrosine
ISSN
:
1319-0989
Journal Name
:
Arts and Humanities Journal
Volume
:
19
Issue Number
:
1
Publishing Year
:
1428 AH
2007 AD
Article Type
:
Article
Added Date
:
Saturday, February 5, 2011
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
صالح عبدالله كابلي
Kabli, Saleh Abdullah
Researcher
Doctorate
Files
File Name
Type
Description
28857.pdf
pdf
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