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Faculty of Medicine
Document Details
Document Type
:
Article In Journal
Document Title
:
Relative quantitation of HIV-1 proviral DNA amplified using the polymerase chain reaction
Relative quantitation of HIV-1 proviral DNA amplified using the polymerase chain reaction
Document Language
:
English
Abstract
:
Abstract: A 141-base pair fragment of human immunodeficiency virus-1 (HIV-1) DNA was amplified using the polymerase chain reaction (PCR). The products were slot-blotted onto a nitrocellulose membrane, revealed with a digoxigenin-labelled probe and quantitated by scanning densitometry. This method for the relative quantitation of HIV-1 DNA achieved reliable results and avoided the use of radioisotopes and the electrophoretic transfer of DNA. Testing of serial dilutions of HIV-1 extracted from infected cells revealed smooth titration curves. A reproducible increase in peripheral blood HIV-1 DNA was documented in a haemophilia patient during disease progression.
ISSN
:
0306-4522
Journal Name
:
Serodiagnosis and Immunotherapy in Infectious Disease
Volume
:
6
Issue Number
:
4
Publishing Year
:
1414 AH
1994 AD
Article Type
:
Article
Added Date
:
Sunday, May 30, 2010
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
آصف جان مان فطاني
Jiman Fatan, Asif
Researcher
Doctorate
Files
File Name
Type
Description
26892.docx
docx
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