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Document Details
Document Type
:
Article In Journal
Document Title
:
Solubilization and Partial Purification of the Plasma Membrane ATPase of Rhodotorula glutinis
الإذابة والتنقية الجزئية لإنزيم الأتيبيز من الغشاء البلازمي لخميرة الرودوتيلا جليتينس
Subject
:
Biochemistry
Document Language
:
English
Abstract
:
The optimal conditions for solubilization of the ATPase activity from the plasma membrane of Rhodotorula glutinis by zwitterionic detergent Z-3-14 were found to be: 0.55 mg/ml (5X cmc) of Z-3-14, at room temperature in the presence of 1 mM ATP, 40% (v/v) glycerol, 11.25% ammonium sulfate, 5 mM 2- mercaptoethanol, 1mM PMSF in 0.1 M Mes-Tris pH 6.5, followed by sonication in a water bath sonicator for 6 min. A 4-fold purification of the solubilized ATPase activity was obtained by centrifugation of the Z-3-14 extract through a glycerol gradient (20-50%, v/v).
ISSN
:
1012-1319
Journal Name
:
Science Journal
Volume
:
17
Issue Number
:
1
Publishing Year
:
1425 AH
2005 AD
Number Of Pages
:
13
Article Type
:
Article
Added Date
:
Sunday, October 11, 2009
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
طه عبدالله قمصاني
TAHA A. KUMOSANI
Researcher
شارلي جرفين
CHARLES C. GRIFFIN
Researcher
Files
File Name
Type
Description
22493.pdf
pdf
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